|
KMID : 0043320100330071035
|
|
Archives of Pharmacal Research
2010 Volume.33 No. 7 p.1035 ~ p.1041
|
|
|
Suppression of adipocyte differentiation by 15-methoxypinusolidic acid through inhibition of PPARγ activity
|
|
Lee Ye-Seul
Sung Sang-Hyun
Hong Jeong-Ho
Hwang Eun-Sook
|
|
|
Abstract
|
|
|
|
Pinusolide and its derivative, 15-methoxypinusolidic acid (15-MPA) are diterpenoid compounds isolated from Biota orientalis, which has been used as a Korean folk medicine for treating inflammatory disorders. Pinusolide and 15-MPA suppress nitric oxide generation by suppressing inducible nitric oxide synthase and exerted anti-inflammatory functions, whereas other functions and regulatory mechanisms are largely unknown. In this study, we investigated whether pinusolide and 15-MPA modulate adipocyte differentiation from pre-adipocytes 3T3-L1 cells. We found that 15-MPA, not pinusolide, suppressed adipocyte differentiation in a dose-dependent manner, as revealed by lipid droplet formation and expression of adipogenic genes such as adiponectin and aP2. 15-MPA did not affect mRNA and protein levels of PPARγ, a key adipogenic transcription factor, whereas transcriptional activity of PPARγ was significantly attenuated by 15-MPA. While aP2 promoter activity was increased by ectopic overexpression of PPARγ or by rosiglitazone-induced endogenous PPARγ activation, PPARγ-induced aP2 promoter activity was inhibited in the presence of 15-MPA. These results suggest that 15-MPA suppresses adipocyte differentiation through the inhibition of PPARγ-dependent adipogenic gene expression.
|
|
|
KEYWORD
|
|
|
Pinusolide, 15-Methoxypinusolidic acid, PPARγ, Adipocyte differentiation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|